New article by Zsolt Karányi et al. has been published in Journal of Cell Biology:
Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation
Zsolt Karányi, László Halász, Laurent Acquaviva, Dávid Jónás, Szabolcs Hetey, Beáta Boros-Oláh, Feng Peng, Doris Chen, Franz Klein, Vincent Géli, Lóránt Székvölgyi
Abstract. Spp1 is the H3K4me3 reader subunit of the Set1 complex (COMPASS/Set1C) that contributes to the mechanism by which meiotic DNA break sites are mechanistically selected. We previously proposed a model in which Spp1 interacts with H3K4me3 and the chromosome axis protein Mer2 that leads to DSB formation. Here we show that spatial interactions of Spp1 and Mer2 occur independently of Set1C. Spp1 exhibits dynamic chromatin binding features during meiosis, with many de novo appearing and disappearing binding sites. Spp1 chromatin binding dynamics depends on its PHD finger and Mer2-interacting domain and on modifiable histone residues (H3R2/K4). Remarkably, association of Spp1 with Mer2 axial sites reduces the effective turnover rate and diffusion coefficient of Spp1 upon chromatin binding, compared with other Set1C subunits. Our results indicate that “chromosomal turnover rate” is a major molecular determinant of Spp1 function in the framework of meiotic chromatin structure that prepares recombination initiation sites for break formation.
New article by Lilla Hornyák et al. has been accepted for publication in Frontiers in Immunology on 17/01/2018:
The role of indoleamine-2,3-dioxygenase (IDO) in cancer development, diagnostics, and therapy
New article by Hetey Sz et al. has been published in Biochemical and Biophysical Research Communications on 21/06/2017. Click here to read!